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The early diagnosis of the acute abdomen is a major goal in surgical practice. Despite the introduction of novel imaging modalities, acute abdominal conditions are still a challenging problem. To date, many inflammatory markers have been used for the diagnosis of acute surgical conditions, including acute appendicitis. Leukocyte count and C-reactive protein (CRP) are the most commonly used laboratory tests. Procalcitonin (PCT) and D-dimer have been studied as novel biomarkers for the diagnosis of acute abdomen in recent years [1].. misuse and dependence can occur in other mental . A convenience sample of emergency department patients aged 18 to 65 years with difficult IV access (2 or more failed landmark attempts) was prospectively randomized to short-axis or long-axis USGPIV. Time from skin puncture to catheter insertion (insertion time) and number of needle sticks were recorded. Number of needle sticks and insertion time were compared using Mann-Whitney U tests.. similarity checked in NCBI Homo sapiens (human) Protein BLAST
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Recombinant adenoviruses were generated using AdEasy system as demonstrated. Briefly, the coding regions of human BMP9 and human CXCL12 were amplified by PCR and subcloned into an adenoviral shuttle vector pAdTrace-TOX and subsequently used to generate recombinant adenoviruses in HEK293 cells. The small interfering RNA (siRNA) target sites against the human CXCR4 coding region were cloned into the homemade siRNA expression pSES adenoviral shuttle vector to generate recombinant adenovirus in HEK293 cells. The resulting adenoviruses were packaged and designated as AdBMP9, Ad-CXCL12and Adsi-CXCR4. Ad-BMP9 expressed green fluorescent protein (GFP), whereas Ad-CXCL12 and Adsi-CXCR4 express red fluorescent protein (RFP) as a marker for monitoring infection efficiency. Analogous adenovirus expressing only monomeric RFP (Ad-RFP) or GFP (Ad-GFP) were used as controls..
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