the laser-cutting pattern for the chamber (Figure 1c) and a laser cutter.
passage in Mus musculus albino mice and in Biomphalaria glabrata. Surgical resections were submitted in fresh to the Pathology Lab within a period of 30 minutes after removal. Handling of specimens was performed following conventional protocols for the management of surgical resections of colon and rectum [26]. Tumor characteristics were recognized on gross examination and selected fragments of tumor were frozen in isopentane and stored at -80ºC. Routine procedures in the Pathology Lab included formalin fixation of the surgical specimen and paraffin embedding of the tissue fragments selected for histopathological examination. Pathological data included in the study are summarized in Table 1. Besides, peripheral venous blood samples from 40 of these patients were collected prior to surgery in EDTA tubes and centrifuged at 1500 rpm during 15 minutes. The obtained plasma was also stored at -80ºC. Enzyme assays have also been performed in plasma obtained from 24 healthy volunteers (matched by sex and age). Surgical resections were submitted in fresh to the Pathology Lab within a period of 30 minutes after removal. Handling of specimens was performed following conventional protocols for the management of surgical resections of colon and rectum [26]. Tumor characteristics were recognized on gross examination and selected fragments of tumor were frozen in isopentane and stored at -80ºC. Routine procedures in the Pathology Lab included formalin fixation of the surgical specimen and paraffin embedding of the tissue fragments selected for histopathological examination. Pathological data included in the study are summarized in Table 1. Besides, peripheral venous blood samples from 40 of these patients were collected prior to surgery in EDTA tubes and centrifuged at 1500 rpm during 15 minutes. The obtained plasma was also stored at -80ºC. Enzyme assays have also been performed in plasma obtained from 24 healthy volunteers (matched by sex and age).. b) Medullary dowels. The clinical features and laboratory findings of the dengue patients are summarized in Table 3. The presence of plasma leakage including ascites, pleural effusion and hemoconcentration distinguished DHF/DSS from DF (p<0.001, Table 3). Tachycardia and restlessness were only detected among the DHF/DSS patients. Presentation of abdominal pain, and hepatomegaly were also statistically associated with DHF/DSS patients in comparison to DF patients (p<0.05). Vomiting, even though not statistically significant (OR=1.7 [95% CI=0.98-2.9], p<0.07), was more frequent in DHF/DSS than in DF. On the other hand, thrombocytopenia (platelet count <100×109/L) and increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (>1000IU/L) were statistically significant in DHF/DSS patients. Leucopenia was common in both DF and DHF/DSS groups..